UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC), Burkholderia cenocepacia, recombinant

Organism: Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)). Source: E.coli. Expression Region: 1-305aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: MLKQRTIKSI VKTVGIGVHS GRKIELTLRP AAPGTGIVFS RVDLPTPVDI PASAMSIGDT RLASVLQKDG VRVSTVEHLM SACAGLGIDN LYVDVTAEEI PIMDGSAATF VFLIQSAGIE EQNAPKRFIK VKKPVEIRDG DKFARLDPYF GFKLKFSIDF RHPAVDKTGQ ELEVDFATTS YVREIARART FGFAHEAEML REIGLARGGS MDNAIVLDEY RILNNDGLRY DDEFVKHKML DAIGDLYVIG HPLLASYTAY KSGHGLNNAL LRELLAHEDA YEIVTFDDPQ AAPKGFAFDA QTAFA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis. Reference: "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients." Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D., Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S., Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K., Scott P., Walker D. Parkhill J. J. Bacteriol. 191:261-277(2009). Function: Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.