Ubiquitin-like modifier-activating enzyme 6 (UBA6), partial, recombinant human

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 43-432aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: LYSRQRYVLG DTAMQKMAKS HVFLSGMGGL GLEIAKNLVL AGIKAVTIHD TEKCQAWDLG TNFFLSEDDV VNKRNRAEAV LKHIAELNPY VHVTSSSVPF NETTDLSFLD KYQCVVLTEM KLPLQKKIND FCRSQCPPIK FISADVHGIW SRLFCDFGDE FEVLDTTGEE PKEIFISNIT QANPGIVTCL ENHPHKLETG QFLTFREING MTGLNGSIQQ ITVISPFSFS IGDTTELEPY LHGGIAVQVK TPKTVFFESL ERQLKHPKCL IVDFSNPEAP LEIHTAMLAL DQFQEKYSRK PNVGCQQDSE ELLKLATSIS ETLEEKPDVN ADIVHWLSWT AQGFLSPLAA AVGGVASQEV LKAVTGKFSP LCQWLYLEAA DIVESLGKPE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. Reference: n/a. Function: nan