Ubiquitin carboxyl-terminal hydrolase 43 (USP43), recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-1123aa. Protein Length: Full Length. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: MDLGPGDAAG GGPLAPRPRR RRSLRRLFSR FLLALGSRSR PGDSPPRPQP GHCDGDGEGG FACAPGPVPA APGSPGEERP PGPQPQLQLP AGDGARPPGA QGLKNHGNTC FMNAVVQCLS NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL VRALWTREYT PQLSAEFKNA VSKYGSQFQG NSQHDALEFL LWLLDRVHED LEGSSRGPVS EKLPPEATKT SENCLSPSAQ LPLGQSFVQS HFQAQYRSSL TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV PILSTVAALR KMVAEEGGVP ADEVILVELY PSGFQRSFFD EEDLNTIAEG DNVYAFQVPP SPSQGTLSAH PLGLSASPRL AAREGQRFSL SLHSESKVLI LFCNLVGSGQ QASRFGPPFL IREDRAVSWA QLQQSILSKV RHLMKSEAPV QNLGSLFSIR VVGLSVACSY LSPKDSRPLC HWAVDRVLHL RRPGGPPHVK LAVEWDSSVK ERLFGSLQEE RAQDADSVWQ QQQAHQQHSC TLDECFQFYT KEEQLAQDDA WKCPHCQVLQ QGMVKLSLWT LPDILIIHLK RFCQVGERRN KLSTLVKFPL SGLNMAPHVA QRSTSPEAGL GPWPSWKQPD CLPTSYPLDF LYDLYAVCNH HGNLQGGHYT AYCRNSLDGQ WYSYDDSTVE PLREDEVNTR GAYILFYQKR NSIPPWSASS SMRGSTSSSL SDHWLLRLGS HAGSTRGSLL SWSSAPCPSL PQVPDSPIFT NSLCNQEKGG LEPRRLVRGV KGRSISMKAP TTSRAKQGPF KTMPLRWSFG SKEKPPGASV ELVEYLESRR RPRSTSQSIV SLLTGTAGED EKSASPRSNV ALPANSEDGG RAIERGPAGV PCPSAQPNHC LAPGNSDGPN TARKLKENAG QDIKLPRKFD LPLTVMPSVE HEKPARPEGQ KAMNWKESFQ MGSKSSPPSP YMGFSGNSKD SRRGTSELDR PLQGTLTLLR SVFRKKENRR NERAEVSPQV PPVSLVSGGL SPAMDGQAPG SPPALRIPEG LARGLGSRLE RDVWSAPSSL RLPRKASRAP RGSALGMSQR TVPGEQASYG TFQRVKYHTL SLGRKKTLPE SSF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Reference: n/a. Function: nan