Ubiquitin carboxyl-terminal hydrolase 30 (Usp30), partial, recombinant mouse

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 57-517aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: TERKKRRKGL VPGLVNLGNT CFMNSLLQGL SACPAFVKWL EEFTTQYSRD QQGPHTHQCL SLTLLNLLKA LSCQEVTEDE VLDASCLLDV LRMYRWQISS FEEQDAHELF HVITSSLEDE RDRQPRVTHL FDVHSLEQQS EMAPRQVTCH TRGSPHPTTN HWKSQHPFHG RLTSNMVCKH CEHQSPVRFD TFDSLSLSIP AATWGHPLTL DHCLHHFISS ESVRDVVCDN CTKIEARGTL TGEKVEHQRS TFVKQLKLGK LPQCLCIHLQ RLSWSSHGTP LKRHEHVQFN EFLMMDFYKY RLLGHKPSQH GPKATENPGS APEVQDAQAA PKPGLSQPGA PKTQIFLNGA CSPSLLPALP SPVAFPLPVV PDYSSSTYLF RLMAVVVHHG DMHSGHFVTY RRSPPSAKNP LSTSNQWLWI SDDTVRKASL QEVLSSSAYL LFYERVLSRV QQQGREYRSE E. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2. Reference: n/a. Function: nan