Ubiquitin carboxyl-terminal hydrolase 19 (Usp19), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 534-1255aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: GFTGLVNLGN TCFMNSVIQS LSNTRELRDF FHDRSFEAEI NYNNPLGTGG RLAIGFAVLL RALWKGTHQA FQPSKLKAIV ASKASQFTGY AQHDAQEFMA FLLDGLHEDL NRIQNKPYTE TVDSDGRPDE VVAEEAWQRH KMRNDSFIVD LFQGQYKSKL VCPVCAKVSI TFDPFLYLPV PLPQKQKVLP IYYFAREPHS KPIKFLVSVS KENSSASEVL ESLSQSVHVK PESLRLAEVI KNRFHRVFLP SHSLDAVSPT DVLLCFELLS PELAKERVVV LEVQQRPQVP SIPISKCAAC QRKQQSEDEK LKRCTRCYRV GYCNQFCQKT HWPDHKGLCR PENIGYPFLV SVPASRLTYA RLAQLLEGYA RYSVSVFQPP FQPGRMALES QSPGCTTLLS TSSLEAGDSE REPIQPSELQ LVTPVAEGDT GAHRMWPPAD RGPVPSTSGI SSEMLASGPM EGCSLLAGER VSRPEAAVPG YQHSRESVSA HTPQFFIYKI DASSREQRLE DKGDTPLELG DDCSLALVWR NNERLQEFVL VASKELECAE DPGSAGEAAR AGHFTLDQCL NLFTRPEVLA PEEAWYCPQC KQHREASKQL LLWRLPNVLI VQLKRFSFRS FIWRDKINDL VEFPVRNLDL SKFCIGQKEE QLPSYDLYAV INHYGGMIGG HYTACARLPS DRSSQRSDVG WRLFDDSTVT TVDESQVVTR YAYVLFYRRR NS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked ubiquitin chains. Reference: n/a. Function: nan