Ubiquitin-associated domain-containing protein 1 (UBAC1) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-405aa. Protein Length: Full Length. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MFVQEEKIFA GKVLRLHICA SDGAEWLEEA TEDTSVEKLK ERCLKHCAHG SLEDPKSITH HKLIHAASER VLSDARTILE ENIQDQDVLL LIKKRAPSPL PKMADVSAEE KKKQDQKAPD KEAILRATAN LPSYNMDRAA VQTNMRDFQT ELRKILVSLI EVAQKLLALN PDAVELFKKA NAMLDEDEDE RVDEAALRQL TEMGFPENRA TKALQLNHMS VPQAMEWLIE HAEDPTIDTP LPGQAPPEAE GATAAASEAA AGASATDEEA RDELTEIFKK IRRKREFRAD ARAVISLMEM GFDEKEVIDA LRVNNNQQNA ACEWLLGDRK PSPEELDKGI DPDSPLFQAI LDNPVVQLGL TNPKTLLAFE DMLENPLNST QWMNDPETGP VMLQISRIFQ TLNRT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 µg/ml can bind human UBAC1, the EC50 of human UBAC1 protein is 86.67-97.25 µg/ml. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle. Reference: n/a. Function: nan