Ubiquilin-1 (UBQLN1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-589aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AESGESGGPP GSQDSAAGAE GAGAPAAAAS AEPKIMKVTV KTPKEKEEFA VPENSSVQQF KEEISKRFKS HTDQLVLIFA GKILKDQDTL SQHGIHDGLT VHLVIKTQNR PQDHSAQQTN TAGSNVTTSS TPNSNSTSGS ATSNPFGLGG LGGLAGLSSL GLNTTNFSEL QSQMQRQLLS NPEMMVQIME NPFVQSMLSN PDLMRQLIMA NPQMQQLIQR NPEISHMLNN PDIMRQTLEL ARNPAMMQEM MRNQDRALSN LESIPGGYNA LRRMYTDIQE PMLSAAQEQF GGNPFASLVS NTSSGEGSQP SRTENRDPLP NPWAPQTSQS SSASSGTAST VGGTTGSTAS GTSGQSTTAP NLVPGVGASM FNTPGMQSLL QQITENPQLM QNMLSAPYMR SMMQSLSQNP DLAAQMMLNN PLFAGNPQLQ EQMRQQLPTF LQQMQNPDTL SAMSNPRAMQ ALLQIQQGLQ TLATEAPGLI PGFTPGLGAL GSTGGSSGTN GSNATPSENT SPTAGTTEPG HQQFIQQMLQ ALAGVNPQLQ NPEVRFQQQL EQLSAMGFLN REANLQALIA TGGDINAAIE RLLGSQPS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:15147878). Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed:19822669, PubMed:18307982). Isoform 1, isoform 2 and isoform 3 play a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress (PubMed:18953672). Involved in the regulation of macroautophagy and autophagosome formation, required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:19148225, PubMed:20529957, PubMed:23459205). Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway (PubMed:21695056). Isoform 1 and isoform 3 play a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis (PubMed:21143716). Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently downregulating the ORAI1-mediated Ca2+ mobilization (PubMed:23307288). Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing (By similarity). Reference: "Ubiquilin interacts with ubiquitylated proteins and proteasome through its ubiquitin-associated and ubiquitin-like domains." Ko H.S., Uehara T., Tsuruma K., Nomura Y. FEBS Lett. 566:110-114(2004). Function: nan