U1 small nuclear ribonucleoprotein A (Snrpa), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-287aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MATIATMPVP ETRANHTIYI NNLNEKIKKD ELKKSLYAIF SQFGQILDIL VSRIMKMRGQ AFVIFKEVTS ATNALRSMQG FPFYDKPMRI QYAKTDSDII AKMKGTYVER DRKREKRKPK SQETPAAKKA VQGGAAAPVV GAVQPVPGMP PMPQAPRIMH HMPGQPPYMP PPGMIPPPGL APGQIPPGAM PPQQLMPGQM PPAQPLSENP PNHILFLTNL PEETNELMLS MLFNQFPGFK EVRLVPGRHD IAFVEFDNEV QAGAARDALQ GFKITQNNAM KISFAKK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs. Reference: "Non-snRNP U1A levels decrease during mammalian B-cell differentiation and release the IgM secretory poly(A) site from repression." Ma J., Gunderson S.I., Phillips C. RNA 12:122-132(2006). Function: nan