U1 small nuclear ribonucleoprotein A (SNRPA), human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 2-282aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: AVPETRPNHT IYINNLNEKI KKDELKKSLY AIFSQFGQIL DILVSRSLKM RGQAFVIFKE VSSATNALRS MQGFPFYDKP MRIQYAKTDS DIIAKMKGTF VERDRKREKR KPKSQETPAT KKAVQGGGAT PVVGAVQGPV PGMPPMTQAP RIMHHMPGQP PYMPPPGMIP PPGLAPGQIP PGAMPPQQLM PGQMPPAQPL SENPPNHILF LTNLPEETNE LMLSMLFNQF PGFKEVRLVP GRHDIAFVEF DNEVQAGAAR DALQGFKITQ NNAMKISFAK K. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assbly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds st loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs. Reference: Structure, chromosomal localization and evolutionary conservation of the gene encoding human U1 snRNP-specific A protein.Nelissen R.L.H., Sillekens P.T.G., Beijer R.P., Geurts van Kessel A.H.M., van Venrooij W.J.Gene 102:189-196(1991). Function: nan