Tyrosine--tRNA ligase, Cytoplasmic domain (YARS1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-528aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal GST-tagged. Target Protein Sequence: GDAPSPEEKL HLITRNLQEV LGEEKLKEIL KERELKIYWG TATTGKPHVA YFVPMSKIAD FLKAGCEVTI LFADLHAYLD NMKAPWELLE LRVSYYENVI KAMLESIGVP LEKLKFIKGT DYQLSKEYTL DVYRLSSVVT QHDSKKAGAE VVKQVEHPLL SGLLYPGLQA LDEEYLKVDA QFGGIDQRKI FTFAEKYLPA LGYSKRVHLM NPMVPGLTGS KMSSSEEESK IDLLDRKEDV KKKLKKAFCE PGNVENNGVL SFIKHVLFPL KSEFVILRDE KWGGNKTYTA YVDLEKDFAA EVVHPGDLKN SVEVALNKLL DPIREKFNTP ALKKLASAAY PDPSKQKPMA KGPAKNSEPE EVIPSRLDIR VGKIITVEKH PDADSLYVEK IDVGEAEPRT VVSGLVQFVP KEELQDRLVV VLCNLKPQKM RGVESQGMLL CASIEGINRQ VEPLDPPAGS APGEHVFVKG YEKGQPDEEL KPKKKVFEKL QADFKISEEC IAQWKQTNFM TKLGSISCKS LKGGNIS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). Reference: Evidence that two present-day components needed for the genetic code appeared after nucleated cells separated from eubacteria.Ribas de Pouplana L., Frugier M., Quinn C.L., Schimmel P.Proc. Natl. Acad. Sci. U.S.A. 93:166-170(1996). Function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction