Tyrosine-protein kinase Mer (Mertk), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Mammalian cell. Expression Region: 19-497aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: GGTAEKEEEI KPDQPFSGPL PGSLPADHRP FFAPHSSGDQ LSPSQTGRSH PAHTATPQMT SAASNLLPPV AFKNTIGRIV LSEHKSVKFN CSINIPNVYQ ETAGISWWKD GKELLGAHHS ITQFYPDEEG VSIIALFSIT SVQRSDNGSY ICKMKVNDRE VVSDPIYVEV QGLPYFTKQP ESVNVTRNTA FNLTCQAVGP PEPVNIFWVQ NSSRVNENPE RSPSVLTVAG LTETAVFSCE AHNDKGLTVS KGVQINIKVI PSPPTEVHIL NSTAHSILVS WVPGFDGYSP LQNCSIQVKE ADQLSNGSVM VFNTSASPHL YEVQQLQALA NYSVTVSCRN EIGWSAVSPW ILASTTEGAP AVAPLNITVF LNESSNNLEI RWTKPPIKRQ DGELVGYRIS HVWESAGTSK ELSEEVSQNG SWAQVPVQMH NATCTVRIAV ITKGGIGPFS EPVDVAIPEH SRVDYAPSST PAPGNTESM. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells. Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Reference: "Mertk triggers uptake of photoreceptor outer segments during phagocytosis by cultured retinal pigment epithelial cells." Feng W., Yasumura D., Matthes M.T., LaVail M.M., Vollrath D. J. Biol. Chem. 277:17016-17022(2002). Function: nan