Troponin I, slow skeletal muscle (Tnni1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 2-187aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-Flag-tagged and C-terminal Myc-tagged. Target Protein Sequence: PEVERKSKIT ASRKLMLKSL MLAKAKECWE QEHEEREAEK VRYLSERIPT LQTRGLSLSA LQDLCRELHA KVEVVDEERY DIEAKCLHNT REIKDLKLKV LDLRGKFKRP PLRRVRVSAD AMLRALLGSK HKVSMDLRAN LKSVKKEDTE KERPVEVGDW RKNVEAMSGM EGRKKMFDAA KSPTSQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Reference: "Large-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos." Neidhardt L., Gasca S., Wertz K., Obermayr F., Worpenberg S., Lehrach H., Herrmann B.G. Mech. Dev. 98:77-94(2000). Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.