Trophoblast glycoprotein (TPBG), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 32-355aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: SSPTSSASSF SSSAPFLASA VSAQPPLPDQ CPALCECSEA ARTVKCVNRN LTEVPTDLPA YVRNLFLTGN QLAVLPAGAF ARRPPLAELA ALNLSGSRLD EVRAGAFEHL PSLRQLDLSH NPLADLSPFA FSGSNASVSA PSPLVELILN HIVPPEDERQ NRSFEGMVVA ALLAGRALQG LRRLELASNH FLYLPRDVLA QLPSLRHLDL SNNSLVSLTY VSFRNLTHLE SLHLEDNALK VLHNGTLAEL QGLPHIRVFL DNNPWVCDCH MADMVTWLKE TEVVQGKDRL TCAYPEKMRN RVLLELNSAD LDCDPILPPS LQTS. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TPBG at 2 µg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC50 is 1.230-1.519 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. Reference: May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. Function: nan