tRNA (guanine-N(7)-)-methyltransferase(Bm1_01445), Brugia malayi, recombinant

Organism: Brugia malayi (Filarial nematode worm). Source: E.coli. Expression Region: 1-258aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MVSTENKIGL FKNKDDDIDG EEMRELPQKK FYRQRAHANP ISDHEFDYPV FPEQMDWKKY FGDFSEGRQV EFADVGCGYG GLLIKLSTLY PEALMVGLEI RVKVSDYVQD KIHALRLREP GNYRNVACLR TNAMKYLPNY FRRHQLTKMF FLYPDPHFKK AKHKWRIITP TLLAEYAYVL KPGGLVYTIT DVEELHIWMV RHLSAHPLFE RLTDLEMKMD PVVEMLYDST EEGQKVARNE GSKWSAVFRR LPNPVLSS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA. Reference: "Draft genome of the filarial nematode parasite Brugia malayi." Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E., Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T., Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L. Scott A.L. Science 317:1756-1760(2007). Function: nan