Trimethylamine-N-oxide reductase 1 (torA), partial, Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 99-572aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RIRYPMVRVD WLRKRHLSDT SQRGDNRFVR VSWDEALDMF YEELERVQKT HGPSALLTAS GWQSTGMFHN ASGMLAKAIA LHGNSVGTGG DYSTGAAQVI LPRVVGSMEV YEQQTSWPLV LQNSKTIVLW GSDLLKNQQA NWWCPDHDVY EYYAQLKAKV AAGEIEVISI DPVVTSTHEY LGREHVKHIA VNPQTDVPLQ LALAHTLYSE NLYDKNFLAN YCVGFEQFLP YLLGEKDGQP KDAAWAEKLT GIDAETIRGL ARQMAANRTQ IIAGWCVQRM QHGEQWAWMI VVLAAMLGQI GLPGGGFGFG WHYNGAGTPG RKGVILSGFS GSTSIPPVHD NSDYKGYSST IPIARFIDAI LEPGKVINWN GKSVKLPPLK MCIFAGTNPF HRHQQINRII EGLRKLETVI AIDNQWTSTC RFADIVLPAT TQFERNDLDQ YGNHSNRGII AMKQVVPPQF EARNDFDIFR ELCR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Reduces trimethylamine-N-oxide (TMAO) into trimethylamine, an anaerobic reaction coupled to energy-yielding reactions. Reference: "Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli." Gon S., Giudici-Orticoni M.-T., Mejean V., Iobbi-Nivol C. J. Biol. Chem. 276:11545-11551(2001). Function: nan