Transmembrane protein PVRIG (PVRIG), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 1-326aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MRTEAQVPAL QPPEPGLEGA MGHRTLVLPW VLLTLCVTAG TPEVWVQVRM EATELSSFTI RCGFLGSGSI SLVTVSWGGP NGAGGTTLAV LHPERGIRQW APARQARWET QSSISLILEG SGASSPCANT TFCCKFASFP EGSWEACGSL PPSSDPGLSA PPTPAPILRA DLAGILGVSG VLLFGCVYLL HLLRRHKHRP APRLQPSRTS PQAPRARAWA PSQASQAALH VPYATINTSC RPATLDTAHP HGGPSWWASL PTHAAHRPQG PAAWASTPIP ARGSFVSVEN GLYAQAGERP PHTGPGLTLF PDPRGPRAME GPLGVR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding. Reference: "Comparative analysis of the paired immunoglobulin-like receptor (PILR) locus in six mammalian genomes: duplication, conversion, and the birth of new genes." Wilson M.D., Cheung J., Martindale D.W., Scherer S.W., Koop B.F. Physiol. Genomics 27:201-218(2006). Function: Cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.