Transmembrane protease serine 6 (Tmprss6), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 81-811aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: KAEVTVSQVY SGSLRVLNRH FSQDLGRRES IAFRSESAKA QKMLQELVAS TRLGTYYNSS SVYSFGEGPL TCFFWFILDI PEYQRLTLSP EVVRELLVDE LLSNSSTLAS YKTEYEVDPE GLVILEASVN DIVVLNSTLG CYRYSYVNPG QVLPLKGPDQ QTTSCLWHLQ GPEDLMIKVR LEWTRVDCRD RVAMYDAAGP LEKRLITSVY GCSRQEPVME VLASGSVMAV VWKKGMHSYY DPFLLSVKSV AFQDCQVNLT LEGRLDTQGF LRTPYYPSYY SPSTHCSWHL TVPSLDYGLA LWFDAYALRR QKYNRLCTQG QWMIQNRRLC GFRTLQPYAE RIPMVASDGV TINFTSQISL TGPGVQVYYS LYNQSDPCPG EFLCSVNGLC VPACDGIKDC PNGLDERNCV CRAMFQCQED STCISLPRVC DRQPDCLNGS DEEQCQEGVP CGTFTFQCED RSCVKKPNPE CDGQSDCRDG SDEQHCDCGL QGLSSRIVGG TVSSEGEWPW QASLQIRGRH ICGGALIADR WVITAAHCFQ EDSMASPKLW TVFLGKMRQN SRWPGEVSFK VSRLFLHPYH EEDSHDYDVA LLQLDHPVVY SATVRPVCLP ARSHFFEPGQ HCWITGWGAQ REGGPVSNTL QKVDVQLVPQ DLCSEAYRYQ VSPRMLCAGY RKGKKDACQG DSGGPLVCRE PSGRWFLAGL VSWGLGCGRP NFFGVYTRVT RVINWIQQVL T. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Through the cleavage of HFE2, a regulator of the expression of the iron absorption-regulating hormone hepicidin/HAMP, plays a role in iron homeostasis. Reference: "Membrane anchored serine proteases: a rapidly expanding group of cell surface proteolytic enzymes with potential roles in cancer." Netzel-Arnett S., Hooper J.D., Szabo R., Madison E.L., Quigley J.P., Bugge T.H., Antalis T.M. Cancer Metastasis Rev. 22:237-258(2003). Function: Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Through the cleavage of HFE2, a regulator of the expression of the iron absorption-regulating hormone hepicidin/HAMP, plays a role in iron homeostasis.