Transient receptor potential cation channel subfamily M member 1 (TRPM1), partial, human, recombinan

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1127-1603aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RCRKKREGDQ EERDRGLKLF LSDEELKRLH EFEEQCVQEH FREKEDEQQS SSDERIRVTS ERVENMSMRL EEINERETFM KTSLQTVDLR LAQLEELSNR MVNALENLAG IDRSDLIQAR SRASSECEAT YLLRQSSINS ADGYSLYRYH FNGEELLFED TSLSTSPGTG VRKKTCSFRI KEEKDVKTHL VPECQNSLHL SLGTSTSATP DGSHLAVDDL KNAEESKLGP DIGISKEDDE RQTDSKKEET ISPSLNKTDV IHGQDKSDVQ NTQLTVETTN IEGTISYPLE ETKITRYFPD ETINACKTMK SRSFVYSRGR KLVGGVNQDV EYSSITDQQL TTEWQCQVQK ITRSHSTDIP YIVSEAAVQA EHKEQFADMQ DEHHVAEAIP RIPRLSLTIT DRNGMENLLS VKPDQTLGFP SLRSKSLHGH PRNVKSIQGK LDRSGHASSV SSLVIVSGMT AEEKKVKKEK ASTETEC. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Forms nonselective divalent cation-conducting channels which mediate the influx of Na(2+), Ca(2+), Mg(2+), Mn(2+), Ba(2+), and Ni(2+) into the cytoplasm, leading to membrane depolarization. Impermeable to zinc ions. In addition, forms heteromultimeric ion channels with TRPM3 which are permeable for calcium and zinc ions. Essential for the depolarizing photoresponse of retinal ON bipolar cells. It is part of the GRM6 signaling cascade. May play a role in metastasis suppression. May act as a spontaneously active, calcium-permeable plasma membrane channel. Reference: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004). Function: nan