Transferrin receptor protein 2 (Tfr2), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 103-798aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RGSCQACGDS VLVVGEDVNS EDSSRGTLYW SDLQDMFLRF LGEGRMEDTI RLTSLRERVA GSARMATLVQ DILDKLSRQK LDHVWTDTHY VGLQFPDPAH PNTLHWVGAD GSVQEQLPLE DPEVYCPYSA TGNATGKLVY AHYGRREDLQ DLKAKDVELA GSLLLVRAGI TSFAQKVAIA QDFGAHGVLI YPDPADFSQD PHKPGLSSDR AVYGHVHLGT GDPYTPGFPS FNQTQFPPVE SSGLPNIPAQ PISADVADRL LRKLTGPVAP QEWKGRLSDS PYRLGPGPGL RLVVNNHRTS TPISNIFACI EGFAEPDHYV VIGAQRDAWG PGAAKSAVGT AILLELVRTF SSMVSSGFRP RRSLLFISWD GGDFGSVGAT EWLEGYLSVL HLKAVVYVSL DNSVLGDGKF HAKTSPLLVS LIENILKQVD SPNHSGQTLY DQVAFTHPSW DAEVIQPLPM DSSAYSFTAF AGVPAVEFSF MEDDRVYPFL HTKEDTYENL HKMLRGRLPA VVLAVAQLAG QLLIRLSHDH LLPLDFGRYG DVVLRHIGNL NEFSGDLKAR GLTLQWVYSA RGDYIRAAEK LRKEIYSSEQ SDERLMRMYN VRIMRVEFYF LSQYVSPADS PFRHIFLGQG DHTLGALVEH LRMLRSDGSG AASSGLSPGL GFQESRFRRQ LALLTWTLQG AANALSGDVW NIDNNF. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Reference: "Comparison of changes in gene expression of transferrin receptor-1 and other iron-regulatory proteins in rat liver and brain during acute-phase response." Malik I.A., Naz N., Sheikh N., Khan S., Moriconi F., Blaschke M., Ramadori G. Cell Tissue Res. 344:299-312(2011). Function: Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation (By similarity).