Transferrin receptor protein 1 (Tfrc), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 89-763aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: CKRVEQKEEC VKLAETEETD KSETMETEDV PTSSRLYWAD LKTLLSEKLN SIEFADTIKQ LSQNTYTPRE AGSQKDESLA YYIENQFHEF KFSKVWRDEH YVKIQVKSSI GQNMVTIVQS NGNLDPVESP EGYVAFSKPT EVSGKLVHAN FGTKKDFEEL SYSVNGSLVI VRAGEITFAE KVANAQSFNA IGVLIYMDKN KFPVVEADLA LFGHAHLGTG DPYTPGFPSF NHTQFPPSQS SGLPNIPVQT ISRAAAEKLF GKMEGSCPAR WNIDSSCKLE LSQNQNVKLI VKNVLKERRI LNIFGVIKGY EEPDRYVVVG AQRDALGAGV AAKSSVGTGL LLKLAQVFSD MISKDGFRPS RSIIFASWTA GDFGAVGATE WLEGYLSSLH LKAFTYINLD KVVLGTSNFK VSASPLLYTL MGKIMQDVKH PVDGKSLYRD SNWISKVEKL SFDNAAYPFL AYSGIPAVSF CFCEDADYPY LGTRLDTYEA LTQKVPQLNQ MVRTAAEVAG QLIIKLTHDV ELNLDYEMYN SKLLSFMKDL NQFKTDIRDM GLSLQWLYSA RGDYFRATSR LTTDFHNAEK TNRFVMREIN DRIMKVEYHF LSPYVSPRES PFRHIFWGSG SHTLSALVEN LKLRQKNITA FNETLFRNQL ALATWTIQGV ANALSGDIWN IDNEF. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Upon stimulation, positively regulates T and B cell proliferation through iron uptake. Reference: "Transferrin receptor is necessary for development of erythrocytes and the nervous system." Levy J.E., Jin O., Fujiwara Y., Kuo F., Andrews N.C. Nat. Genet. 21:396-399(1999). Function: nan