TPR repeat-containing thioredoxin TDX (Os09g0401200), Oryza sativa subsp. japonica, recombinant

Organism: Oryza sativa subsp. japonica (Rice). Source: E.coli. Expression Region: 1-317aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MATAGASSFE DEIMESDIEL EGEAVEPDND PPQKMGDPSV EVSDEKRDQA QLCKNKGVDA FSEGKLDEAI EHLTEAIVLN PTSAIAYATR AVIFVKSKKP NAAIRDADAA LKINPDSAKG YKSRGMAKAM LGKWEEAAQD LRMAAKLDYD EEIGAELKKV EPNVLKIEEH RKKYERLRKE RDIKKAEMEK QRKHAEEVSA ASAALKDGDV IAIHSSSELD TKLKAASSLS RLVVLYFTAA WCGPCRFIGP VCKSLAEKHR NVVFLKVDID ELNSVAYRWN VSSVPSFFFV RNGKEIDKVV GADKNGLERK VAQHGSS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Probable thiol-disulfide oxidoreductase that may participate in various redox reactions and act as chaperone under heat shock. May interact with HSP70 proteins through the TPR repeats. Reference: "Comparative genomic study of the thioredoxin family in photosynthetic organisms with emphasis on Populus trichocarpa." Chibani K., Wingsle G., Jacquot J.P., Gelhaye E., Rouhier N. Mol. Plant 2:308-322(2009). Function: Probable thiol-disulfide oxidoreductase that may participate in various redox reactions and act as chaperone under heat shock. May interact with HSP70 proteins through the TPR repeats (By similarity).