Tomoregulin-1 (TMEFF1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 40-330aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SNQPPGGGGG SGGDCPGGKG KSINCSELNV RESDVRVCDE SSCKYGGVCK EDGDGLKCAC QFQCHTNYIP VCGSNGDTYQ NECFLRRAAC KHQKEITVIA RGPCYSDNGS GSGEGEEEGS GAEVHRKHSK CGPCKYKAEC DEDAENVGCV CNIDCSGYSF NPVCASDGSS YNNPCFVREA SCIKQEQIDI RHLGHCTDTD DTSLLGKKDD GLQYRPDVKD ASDQREDVYI GNHMPCPENL NGYCIHGKCE FIYSTQKASC RCESGYTGQH CEKTDFSILY VVPSRQKLTH V. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May inhibit NODAL and BMP signaling during neural patterning. May be a tumor suppressor in brain cancers. Reference: "A novel transmembrane protein with epidermal growth factor and follistatin domains expressed in the hypothalamo-hypophysial axis of Xenopus laevis." Eib D.W., Martens G.J.M. J. Neurochem. 67:1047-1055(1996). Function: May inhibit NODAL and BMP signaling during neural patterning (By similarity). May be a tumor suppressor in brain cancers.