Thyrotropin receptor (Tshr), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 22-764aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: RGCTSPPCEC HQEDDFRVTC KELHQIPSLP PSTQTLKLIE THLKTIPSLA FSSLPNISRI YLSIDATLQR LEPHSFYNLS KMTHIEIRNT RSLTYIDPDA LTELPLLKFL GIFNTGLRIF PDLTKIYSTD VFFILEITDN PYMTSVPENA FQGLCNETLT LKLYNNGFTS IQGHAFNGTK LDAVYLNKNK YLTAIDKDAF GGVYSGPTLL DVSSTSVTAL PSKGLEHLKE LIAKNTWTLK KLPLSLSFLH LTRADLSYPS HCCAFKNQKK IRGILESLMC NESSIRNLRQ RKSVNVMRGP VYQEYEEGLG DNHVGYKQNS KFQEGPSNSH YYVFFEEQED EIIGFGQELK NPQEETLQAF DSHYDYTVCG DNEDMVCTPK SDEFNPCEDI MGYKFLRIVV WFVSPMALLG NVFVLFVLLT SHYKLTVPRF LMCNLAFADF CMGVYLLLIA SVDLYTHTEY YNHAIDWQTG PGCNTAGFFT VFASELSVYT LTVITLERWY AITFAMRLDR KIRLRHAYTI MAGGWVSCFL LALLPMVGIS SYAKVSICLP MDTDTPLALA YIALVLLLNV VAFVIVCSCY VKIYITVRNP QYNPRDKDTK IAKRMAVLIF TDFMCMAPIS FYALSALMNK PLITVTNSGV LLVLFYPLNS CANPFLYAIF TKAFQRDVFI LLSKFGLCKH QAQAYQAQRV CPNNNTGIQI QKIPQDTRQS LPNVQDTYEP LGSSHLTPKL QGRISEEYTQ TAL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism. Reference: n/a. Function: nan