Tetanus toxin (tetX), partial, Clostridium tetani, recombinant

Organism: Clostridium tetani (strain Massachusetts / E88). Source: E.coli. Expression Region: 2-457aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-B2M-tagged. Target Protein Sequence: PITINNFRYS DPVNNDTIIM MEPPYCKGLD IYYKAFKITD RIWIVPERYE FGTKPEDFNP PSSLIEGASE YYDPNYLRTD SDKDRFLQTM VKLFNRIKNN VAGEALLDKI INAIPYLGNS YSLLDKFDTN SNSVSFNLLE QDPSGATTKS AMLTNLIIFG PGPVLNKNEV RGIVLRVDNK NYFPCRDGFG SIMQMAFCPE YVPTFDNVIE NITSLTIGKS KYFQDPALLL MHELIHVLHG LYGMQVSSHE IIPSKQEIYM QHTYPISAEE LFTFGGQDAN LISIDIKNDL YEKTLNDYKA IANKLSQVTS CNDPNIDIDS YKQIYQQKYQ FDKDSNGQYI VNEDKFQILY NSIMYGFTEI ELGKKFNIKT RLSYFSMNHD PVKIPNLLDD TIYNDTEGFN IESKDLKSEY KGQNMRVNTN AFRNVDGSGL VSKLIGLCKK IIPPTNIREN LYNRTA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-, -Phe-77' bond of synaptobrevin-2. Reference: "Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins." Eisel U., Jarausch W., Goretzki K., Henschen A., Engels J., Weller U., Hudel M., Habermann E., Niemann H. EMBO J. 5:2495-2502(1986). Function: Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-, -Phe-77' bond of synaptobrevin-2.