Tetanus toxin, partial, Clostridium tetani, recombinant

Organism: Clostridium tetani. Source: E.coli. Expression Region: 459-865aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: ALTDLGGELC IKIKNEDLIF IAEKNSFSEE PFQDEIVSYN TKNKPLNFNY SLDKIILDYN LQSKITLPND RTTPVTKGIP YAPEYKSNAA STIEIHNIDD NTIYQYLYAQ KSPTTLQRIT MTNSVDDALI NSTKIYSYFP SVISKVNQGA QGILFLQWVR DIIDDFTNES SQKTTIDKIS DVSTIVPYIG PALNIVKQGY EGNFIGALET TGVVLLLEYI PEITLPVIAA LSIAESSTQK EKIIKTIDNF LEKRYEKWIE VYKLVKAKWL GTVNTQFQKR SYQMYRSLEY QVDAIKKIID YEYKIYSGPD KEQIADEINN LKNKLEEKAN KAMININIFM RESSRSFLVN QMINETKKQL LEFDTQSKNI LMQYIKANSK FIGITELKKL ESKINKVFST PIPFSYS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Cloning and sequence analysis of tetanus toxin gene." Shumin Z., Dianliang L. Submitted (JUN-2001). Function: nan