Terminal nucleotidyltransferase 4B (TENT4B), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-572aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MYRSGERLLG SHALPAEQRD FLPLETTNNN NNHHQPGAWA RRAGSSASSP PSASSSPHPS AAVPAADPAD SASGSSNKRK RDNKASGGRA AGGGRADGGG VVYSGTPWKR RNYNQGVVGL HEEISDFYEY MSPRPEEEKM RMEVVNRIES VIKELWPSAD VQIFGSFKTG LYLPTSDIDL VVFGKWENLP LWTLEEALRK HKVADEDSVK VLDKATVPII KLTDSFTEVK VDISFNVQNG VRAADLIKDF TKKYPVLPYL VLVLKQFLLQ RDLNEVFTGG IGSYSLFLMA VSFLQLHPRE DACIPNTNYG VLLIEFFELY GRHFNYLKTG IRIKDGGSYV AKDEVQKNML DGYRPSMLYI EDPLQPGNDV GRSSYGAMQV KQAFDYAYVV LSHAVSPIAK YYPNNETESI LGRIIRVTDE VATYRDWISK QWGLKNRPEP SCNGPVSSSS ATQSSSSDVD SDATPCKTPK QLLCRPSTGN RVGSQDVSLE SSQAVGKMQS TQTTNTSNST NKSQHGSARL FRSSSKGFQG TTQTSHGSLM TNKQHQGKSN NQYYHGKKRK HKRDAPLSDL CR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalytic subunit of a TRAMP-like complex which has a poly RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity. Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1. Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion. Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation. Mediates 3' adenylation of H/ACA box snoRNAs followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation. Reference: "Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent adenylation and PARN-dependent trimming." Berndt H., Harnisch C., Rammelt C., Stoehr N., Zirkel A., Dohm J.C., Himmelbauer H., Tavanez J.P., Huettelmaier S., Wahle E. RNA 18:958-972(2012). Function: Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion.