Telomerase, Yeast, Control Peptide (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-asso

A 23aa Peptide sequence mapping near the N-terminus of yeast telomerase/Est2 (1) was selected for antibody production. The peptide was coupled to KLH. The 3'ends of chromosomes are capped with telomere sequences (TTAGGG, 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities. Most recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling. (2). , Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2, hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa, mouse Est2 1122 aa, ~127kD). hEST2 was localized in the nucleus. Yeast telomerase is 988 aa protein. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.