TelN Protelomerase

Product Description: TelN Protelomerase is cloned from bacteriophage N15. TelN cuts dsDNA at the recognition site TelRL (56 bp), which consists of a palindromic sequence-TelO in the middle and palindromic sequences R3 and L3 at both ends of 14 bp form. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini. Specifications: Synonyms: TelN, Phage N15 protelomerase, Protelomerase, TelN enzyme, TelN protein. Application: In vitro enzymatic synthesis of DNA constructs. Concentration: 10U/µL. Unit Definition: One unit is defined as the amount of enzyme required to cut 0.5 µg completely BsaI linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) at 30 °C for 30 min in a total reaction volume of 20 µL. Source: E.coli strain that carries TelN from phage N15.