T7 RNA Polymerase

T7 RNA polymerase is a recombinant enzyme purified from Escherichia coli, for the synthesis of high specific-activity RNA probes, biologically active mRNA and antisense RNA. T7 RNA polymerase is DNA-dependent with strict specificity for its own double-stranded promoter that is not efficiently recognized by SP6 or T3 RNA polymerases. T7 RNA polymerase catalyzes the 5'->3' synthesis of RNA from ribonucleoside triphosphates on single or double-stranded DNA downstream from a T7 promoter. Using circular plasmid DNA as a template will result in heterogeneous transcripts of multiple lengths. T7 RNA polymerase accepts modified nucleotides as substrates for RNA synthesis. Components: * T7 RNA Polymerase (20 U/µL or 200 U/µL) * Reaction Buffer (10x). Shipping Conditions: Dry Ice. Storage Conditions: -85 °C to -15 °C. Application: In vitro RNA synthesis. Format: Enzyme/Protein, Liquid. Product Category: RNA Synthesis. Reaction Temperature: 37 °C. Heat Inactivation: 70 °C. Protocol Time: Approx. 120 min