Supreme NZYLong DNA polymerase

Supreme NZYLong DNA polymerase is an engineered version of NZYLong DNA polymerase designed to amplify longer PCR products, generally of 25 kb and beyond, which displays a higher specificity provided by its hot-start-like PCR capacity. This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3'->5' exonuclease activities and thus avoids extension of nonspecifically annealed primers or primer-dimers, as well as degradation of primers and template DNA during PCR reaction setup. The functional activity of the enzyme is restored during a short 5-minute incubation step at 94 °C. The increased processivity of Supreme NZYLong DNA polymerase combined with the hot-startlike PCR capacity results in higher specificity, sensitivity and yield during the amplification of long nucleic acids. A wide range of long PCR products, typically of 25 kb and up to 40 kb, can be generated usimg lambda DNA or human genomic DNA as starting template. Supreme NZYLong DNA polymerase displays a higher fidelity than conventional Taq DNA polymerases. The provided 10x reaction buffer protects DNA from depurination and nicking during long thermal cycling. The enzyme generates a mixture of A-overhangended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYTech's TA PCR cloning kits (MB053 or MB137).Extension time: 60 sec/kb at 68 °C Product overhang: mixed. Components: * Supreme NZYLong DNA polymerase (5 U/µL) * Reaction buffer (10x). Shipping Conditions: Room Temperature. Storage Conditions: -85°C to -15°C. Application: Routine PCR, TA Cloning. Features: Hot-start, Long-range. Product Length: 0-20 kb. Format: Enzyme/Protein. Product Category: End-Point PCR. Concentration: 5 U/µL. Hot Start: Yes