Sucrase-isomaltase, intestinal (SI), partial, recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1008-1827aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: IKLPSDPIST LRVEVKYHKN DMLQFKIYDP QKKRYEVPVP LNIPTTPIST YEDRLYDVEI KENPFGIQIR RRSSGRVIWD SWLPGFAFND QFIQISTRLP SEYIYGFGEV EHTAFKRDLN WNTWGMFTRD QPPGYKLNSY GFHPYYMALE EEGNAHGVFL LNSNAMDVTF QPTPALTYRT VGGILDFYMF LGPTPEVATK QYHEVIGHPV MPAYWALGFQ LCRYGYANTS EVRELYDAMV AANIPYDVQY TDIDYMERQL DFTIGEAFQD LPQFVDKIRG EGMRYIIILD PAISGNETKT YPAFERGQQN DVFVKWPNTN DICWAKVWPD LPNITIDKTL TEDEAVNASR AHVAFPDFFR TSTAEWWARE IVDFYNEKMK FDGLWIDMNE PSSFVNGTTT NQCRNDELNY PPYFPELTKR TDGLHFRTIC MEAEQILSDG TSVLHYDVHN LYGWSQMKPT HDALQKTTGK RGIVISRSTY PTSGRWGGHW LGDNYARWDN MDKSIIGMME FSLFGMSYTG ADICGFFNNS EYHLCTRWMQ LGAFYPYSRN HNIANTRRQD PASWNETFAE MSRNILNIRY TLLPYFYTQM HEIHANGGTV IRPLLHEFFD EKPTWDIFKQ FLWGPAFMVT PVLEPYVQTV NAYVPNARWF DYHTGKDIGV RGQFQTFNAS YDTINLHVRG GHILPCQEPA QNTFYSRQKH MKLIVAADDN QMAQGSLFWD DGESIDTYER DLYLSVQFNL NQTTLTSTIL KRGYINKSET RLGSLHVWGK GTTPVNAVTL TYNGNKNSLP FNEDTTNMIL RIDLTTHNVT LEEPIEINWS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Plays a critical role in cytoplasmic virus egress. Participates in the final step of tegumentation and envelope acquisition within the host cytoplasm by directly interacting with the capsid. Upon virion binding to target cell, a signaling cascade is triggered to disrupt the interaction with the capsid, thereby preparing capsid uncoating. Reference: "Epstein-Barr virus and virus human protein interaction maps." Calderwood M.A., Venkatesan K., Xing L., Chase M.R., Vazquez A., Holthaus A.M., Ewence A.E., Li N., Hirozane-Kishikawa T., Hill D.E., Vidal M., Kieff E., Johannsen E. Proc Natl Acad Sci U S A 104:7606-7611(2007). Function: nan