Succinyl-CoA synthetase

Succinyl-CoA synthetase from E. coli is responsible for the coupled hydrolysis of succinyl-CoA to the production of ATP (or GTP) in the substrate-level phosphorylation step of the citric acid cycle (TCA). This enzyme complex is formed as a heterotetramer, alpha2beta2, where the two alpha subunits each contain the coenzyme A and phosphate binding sites, and the two beta subunits each contain the succinate binding sites and provide nucleotide binding specificity. This enzyme is Mg2+-dependent.Synonyms: SCS, Succinate Thiokinase, Succinyl Coenzyme A Synthetase. Purity: >85% estimated by SDS-PAGE. Source: Active E. coli (strain K12) C-terminal His-tagged Succinyl-CoA synthetase enzyme purified from E. coli. MW: 29.8 kDa (alpha), 41.4 kDa (beta). Formulation: (Request formulation change), 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 20% glycerol. UniProt Accession #: P0A836, P0AGE9.