Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (ERG26), Saccharomyces cerevisiae, recom

Organism: SVG-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Source: E.coli. Expression Region: 1-349aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MSKIDSVLII GGSGFLGLHL IQQFFDINPK PDIHIFDVRD LPEKLSKQFT FNVDDIKFHK GDLTSPDDME NAINESKANV VVHCASPMHG QNPDIYDIVN VKGTRNVIDM CKKCGVNILV YTSSAGVIFN GQDVHNADET WPIPEVPMDA YNETKAIAED MVLKANDPSS DFYTVALRPA GIFGPGDRQL VPGLRQVAKL GQSKFQIGDN NNLFDWTYAG NVADAHVLAA QKLLDPKTRT AVSGETFFIT NDTPTYFWAL ARTVWKADGH IDKHVIVLKR PVAICAGYLS EWVSKMLGKE PGLTPFRVKI VCAYRYHNIA KAKKLLGYTP RVGIEEGINK TLAWMDEGL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Present with 2580 molecules/cell in log phase SD medium. Reference: "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003). Function: nan