Sprouty-related, EVH1 domain-containing protein 1 (SPRED1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-444aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: SEETATSDND NSYARVRAVV MTRDDSSGGW LPLGGSGLSS VTVFKVPHQE ENGCADFFIR GERLRDKMVV LECMLKKDLI YNKVTPTFHH WKIDDKKFGL TFQSPADARA FDRGIRRAIE DISQGCPESK NEAEGADDLQ ANEEDSSSSL VKDHLFQQET VVTSEPYRSS NIRPSPFEDL NARRVYMQSQ ANQITFGQPG LDIQSRSMEY VQRQISKECG SLKSQNRVPL KSIRHVSFQD EDEIVRINPR DILIRRYADY RHPDMWKNDL ERDDADSSIQ FSKPDSKKSD YLYSCGDETK LSSPKDSVVF KTQPSSLKIK KSKRRKEDGE RSRCVYCQER FNHEENVRGK CQDAPDPIKR CIYQVSCMLC AESMLYHCMS DSEGDFSDPC SCDTSDDKFC LRWLALVALS FIVPCMCCYV PLRMCHRCGE ACGCCGGKHK AAG. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Reference: "Distinct requirements for the Sprouty domain for functional activity of Spred proteins." King J.A.J., Straffon A.F.L., D'Abaco G.M., Poon C.L.C., I S.T.T., Smith C.M., Buchert M., Corcoran N.M., Hall N.E., Callus B.A., Sarcevic B., Martin D., Lock P., Hovens C.M. Biochem. J. 388:445-454(2005). Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity).