Spike glycoprotein (S), partial, recombinant human coronavirus NL63

Organism: Human coronavirus NL63 (HCoV-NL63). Source: Mammalian cell. Expression Region: 19-717aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: CNSNANLSML QLGVPDNSST IVTGLLPTHW FCANQSTSVY SANGFFYIDV GNHRSAFALH TGYYDANQYY IYVTNEIGLN ASVTLKICKF SRNTTFDFLS NASSSFDCIV NLLFTEQLGA PLGITISGET VRLHLYNVTR TFYVPAAYKL TKLSVKCYFN YSCVFSVVNA TVTVNVTTHN GRVVNYTVCD DCNGYTDNIF SVQQDGRIPN GFPFNNWFLL TNGSTLVDGV SRLYQPLRLT CLWPVPGLKS STGFVYFNAT GSDVNCNGYQ HNSVVDVMRY NLNFSANSLD NLKSGVIVFK TLQYDVLFYC SNSSSGVLDT TIPFGPSSQP YYCFINSTIN TTHVSTFVGI LPPTVREIVV ARTGQFYING FKYFDLGFIE AVNFNVTTAS ATDFWTVAFA TFVDVLVNVS ATNIQNLLYC DSPFEKLQCE HLQFGLQDGF YSANFLDDNV LPETYVALPI YYQHTDINFT ATASFGGSCY VCKPHQVNIS LNGNTSVCVR TSHFSIRYIY NRVKSGSPGD SSWHIYLKSG TCPFSFSKLN NFQKFKTICF STVEVPGSCN FPLEATWHYT SYTIVGALYV TWSEGNSITG VPYPVSGIRE FSNLVLNNCT KYNIYDYVGT GIIRSSNQSL AGGITYVSNS GNLLGFKNVS TGNIFIVTPC NQPDQVAVYQ QSIIGAMTAV NESRYGLQNL LQLPNFYYV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: S1 region attaches the virion to the cell membrane by interacting with host ACE2, initiating the infection (PubMed:15897467, PubMed:19901337, PubMed:29142129). Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Reference: n/a. Function: nan