Spike glycoprotein (S), partial, human coronavirus 229E, recombinant

Organism: Human coronavirus 229E (HCoV-229E). Source: Mammalian cell. Expression Region: 16-547aa. Protein Length: Partial (S1 Domain). Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: CQTTNGLNTS YSVCNGCVGY SENVFAVESG GYIPSDFAFN NWFLLTNTSS VVDGVVRSFQ PLLLNCLWSV SGLRFTTGFV YFNGTGRGDC KGFSSDVLSD VIRYNLNFEE NLRRGTILFK TSYGVVVFYC TNNTLVSGDA HIPFGTVLGN FYCFVNTTIG NETTSAFVGA LPKTVREFVI SRTGHFYING YRYFTLGNVE AVNFNVTTAE TTDFCTVALA SYADVLVNVS QTSIANIIYC NSVINRLRCD QLSFDVPDGF YSTSPIQSVE LPVSIVSLPV YHKHTFIVLY VDFKPQSGGG KCFNCYPAGV NITLANFNET KGPLCVDTSH FTTKYVAVYA NVGRWSASIN TGNCPFSFGK VNNFVKFGSV CFSLKDIPGG CAMPIVANWA YSKYYTIGSL YVSWSDGDGI TGVPQPVEGV SSFMNVTLDK CTKYNIYDVS GVGVIRVSND TFLNGITYTS TSGNLLGFKD VTKGTIYSIT PCNPPDQLVV YQQAVVGAML SENFTSYGFS NVVELPKFFY ASNGTYNCTD AV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Lyophilized powder. Buffer: Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: n/a. Function: nan