Spike glycoprotein (S), partial, Feline coronavirus, recombinant

Organism: Feline coronavirus (strain FIPV WSU-79/1146) (FCoV). Source: E.coli. Expression Region: 561-804aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: PMQDNNTDVY CIRSNQFSVY VHSTCKSSLW DNIFNQDCTD VLEATAVIKT GTCPFSFDKL NNYLTFNKFC LSLSPVGANC KFDVAARTRT NEQVVRSLYV IYEEGDNIVG VPSDNSGLHD LSVLHLDSCT DYNIYGRTGV GIIRRTNSTL LSGLYYTSLS GDLLGFKNVS DGVIYSVTPC DVSAQAAVID GAIVGAMTSI NSELLGLTHW TTTPNFYYYS IYNYTSERTR GTAIDSNDVD CEPV. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Reference: n/a. Function: nan