SpCas9 D10A Nickase

Product Description: Recombinant SpCas9 D10A Nickase, from Streptococcus pyogenes, a mutant version of Cas9, Cas9 nickase (Cas9n), generates single-strand nicks in DNA. Paired nickases, which effectively create DSBs by generating two single-strand breaks a short distance apart at a targeted site, have been introduced to reduce such off-target activity. Concentration: 10mg/ml. Source: E. coli with the Cas9 mutant (D10A) gene from Streptococcus pyogenes. Purity: > 95%. Endotoxin: <=10 EU/mg. Quality Standards: Purity (Bis-Tris Page): > 95%, Purity (SEC-HPLC): > 95%, Purity (RP-HPLC): > 95%, Endotoxin: <=10 EU/mg.