Soluble interferon gamma receptor B8 (B8R), Vaccinia virus, recombinant

Organism: Vaccinia virus (strain Copenhagen) (VACV). Source: Mammalian cell. Expression Region: 14-272aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: SIHAKITSYK FESVNFDSKI EWTGDGLYNI SLKNYGIKTW QTMYTNVPEG TYDISAFPKN DFVSFWVKFE QGDYKVEEYC TGLCVEVKIG PPTVTLTEYD DHINLYIEHP YATRGSKKIP IYKRGDMCDI YLLYTANFTF GDSEEPVTYD IDDYDCTSTG CSIDFATTEK VCVTAQGATE GFLEKITPWS SEVCLTPKKN VYTCAIRSKE DVPNFKDKMA RVIKRKFNKQ SQSYLTKFLG STSNDVTTFL SMLNLTKYS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Counteracts the antiviral effects of host IFN-gamma. Acts as a soluble IFN-gamma receptor and thus inhibits the interaction between host IFN-gamma and its receptor. Reference: "The complete DNA sequence of vaccinia virus." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:247-266(1990). Function: nan