Soluble calcium-activated nucleotidase 1 (CANT1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 80-397aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: GQAPANWYND TYPLSPPQRT PAGIRYRIAV IADLDTESRA QEENTWFSYL KKGYLTLSDS GDKVAVEWDK DHGVLESHLA EKGRGMELSD LIVFNGKLYS VDDRTGVVYQ IEGSKAVPWV ILSDGDGTVE KGFKAEWLAV KDERLYVGGL GKEWTTTTGD VVNENPEWVK VVGYKGSVDH ENWVSNYNAL RAAAGIQPPG YLIHESACWS DTLQRWFFLP RRASQERYSE KDDERKGANL LLSASPDFGD IAVSHVGAVV PTHGFSSFKF IPNTDDQIIV ALKSEEDSGR VASYIMAFTL DGRFLLPETK IGSVKYEG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis. Reference: Cloning, expression, and characterization of a soluble calcium-activated nucleotidase, a human enzyme belonging to a new family of Extracellular domain nucleotidases.Smith T., Hicks-Berger C., Kim S., Kirley T.Arch. Biochem. Biophys. 406:105-115(2002). Function: Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP