Signal-Regulatory Protein alpha-1/SIRPA/CD172a (C-6His) (recombinant cynomolgus)

Activity: Immobilized Cynomolgus SIRPA-His(Cat#PKSQ050105) at 10µg/ml (100 µl/well) can bind Anti-Human SIRPA mAb-Fc. The ED50 of Anti-Human SIRPA mAb-Fc is 17.7 ng/ml. Protein Construction: Recombinant Cynomolgus Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Glu31­Arg369 is expressed with a 6His tag at the C-terminus. Sequence: Glu31­Arg369. Fusion tag: C-6His Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 45-65 kDa. Protein function: Signal Regulatory Protein alpha (SIRPalpha) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPalpha contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPalpha can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPalpha acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPalpha shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPalpha engagement generally produces a negative regulatory signal, it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation