Siglec-E Protein, Mouse, Recombinant (hFc)

Description: Siglecs are sialic acid specific Iâ??type lectins that are characterized by an extracellular domain (ECD) with an Nâ??terminal Igâ??like Vâ??set domain followed by varying numbers of Igâ??like C2â??set domains. Mouse Siglecâ??E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 â?? 85 kDa member of the CD33â??related subfamily of Siglecs. Rodent and primate Siglec gene families have significantly diverged, and Siglecâ??9 is the most likely human ortholog of mouse Siglecâ??E. Siglecâ??E is expressed as a heavily Nâ??glycosylated disulfideâ??linked homodimer and shows binding preference for disialic acids in the alpha 2â??8 linkage. Siglecâ??E is upâ??regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists. Siglecâ??E signaling negatively regulates the LPSâ??induced production of TNFâ?? alpha and ILâ??6 by macrophages. Its upâ??regulation in macrophages parallels the development of endotoxin tolerance. Siglecâ??E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell ILâ??12 p40 production.