Sialidase-4 (Neu4), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 1-478aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MGPTRVPRRT VLFQRERTGL TYRVPALLCV PPRPTLLAFA EQRLSPDDSH AHRLVLRRGT LTRGSVRWGT LSVLETAVLE EHRSMNPCPV LDEHSGTIFL FFIAVLGHTP EAVQIATGKN AARLCCVTSC DAGLTWGSVR DLTEEAIGAA LQDWATFAVG PGHGVQLRSG RLLVPAYTYH VDRRECFGKI CWTSPHSLAF YSDDHGISWH CGGLVPNLRS GECQLAAVDG DFLYCNARSP LGNRVQALSA DEGTSFLPGE LVPTLAETAR GCQGSIVGFL APPSIEPQDD RWTGSPRNTP HSPCFNLRVQ ESSGEGARGL LERWMPRLPL CYPQSRSPEN HGLEPGSDGD KTSWTPECPM SSDSMLQSPT WLLYSHPAGR RARLHMGIYL SRSPLDPHSW TEPWVIYEGP SGYSDLAFLG PMPGASLVFA CLFESGTRTS YEDISFCLFS LADVLENVPT GLEMLSLRDK AQGHCWPS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May function in lysosomal catabolism of sialylated glycoconjugates. Has sialidase activity towards synthetic substrates, such as 2'--alpha-D-N-acetylneuraminic acid . Has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids . Reference: "Identification and expression of Neu4, a novel murine sialidase." Comelli E.M., Amado M., Lustig S.R., Paulson J.C. Gene 321:155-161(2003). Function: nan