Sialidase 33A, Bacteroides fragilis

Sialidase 33A (BfNan33A), assigned the E.C. number 3.2.1.18, is a derivative of Bacteroides fragilis. It is an exo-alpha-sialidase. The recombinant BfNan33A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 33 (GH33) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Sialidases. Family: GH33. Ec Number: 3.2.1.18. Organism: Bacteroides fragilis. Architecture: GH33. Optimal pH: 6.5. Optimal Temperature: 37°C. Substrates: colominic acid, glycolipids, glycoproteins, sialic acids, synthetic substrates