Shiga-like toxin 2 subunit A (stxA2) (E189K), recombinant Enterobacteria phage 933W

Organism: Enterobacteria phage 933W (Bacteriophage 933W). Source: E.coli. Expression Region: 23-319aa(E189K). Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: REFTIDFSTQ QSYVSSLNSI RTEISTPLEH ISQGTTSVSV INHTPPGSYF AVDIRGLDVY QARFDHLRLI IEQNNLYVAG FVNTATNTFY RFSDFTHISV PGVTTVSMTT DSSYTTLQRV AALERSGMQI SRHSLVSSYL ALMEFSGNTM TRDASRAVLR FVTVTAKALR FRQIQREFRQ ALSETAPVYT MTPGDVDLTL NWGRISNVLP EYRGEDGVRV GRISFNNISA ILGTVAVILN CHHQGARSVR AVNEESQPEC QITGDRPVIK INNTLWESNT AAAFLNRKSQ FLYTTGK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits. Catalytic activity Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. EC:3.2.2.22. Reference: "Phylogenetic diversity and similarity of active sites of Shiga toxin (stx) in Shiga toxin-producing Escherichia coli (STEC) isolates from humans and animals." Asakura H., Makino S., Kobori H., Watarai M., Shirahata T., Ikeda T., Takeshi K. Epidemiol. Infect. 127:27-36(2001). Function: nan