Serum paraoxonase/arylesterase 1 (Pon1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 2-355aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: AKLLALTLVG LVLALYKNHR SSYQTRLNAF REVTPVELPN CNLVKGIETG AEDLEILPNG LTFFSTGLKY PGIKSFDPSK PGKILLMDLN KKEPAVSELE IIGNTLDISS FNPHGISTFT DEDNTVYLLV VNHPDSSSTV EVFKFQEEER SLLHLKTITH ELLPSINDIA AIGPESFYAT NDHYFADPYL RSWEMYLGLS WSNVVYYSPD KVQVVAEGFD FANGIGISLD GKYVYIAELL AHKIHVYEKH ANWTLTPLKV LNFDTLVDNI SVDPVTGDLW VGCHPNGMRI FFYDAENPPG SEVLRIQNIL SEDPKITVVY AENGTVLQGT TVASVYKGKL LIGTVFHKAL YCDL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification. Reference: "Targeted disruption of the murine lecithin:cholesterol acyltransferase gene is associated with reductions in plasma paraoxonase and platelet-activating factor acetylhydrolase activities but not in apolipoprotein J concentration." Forte T.M., Oda M.N., Knoff L., Frei B., Suh J., Harmony J.A., Stuart W.D., Rubin E.M., Ng D.S. J. Lipid Res. 40:1276-1283(1999). Function: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.