Serum albumin (ALB), dog, recombinant

Organism: Canis lupus familiaris (Dog) (Canis familiaris). Source: Yeast. Expression Region: 25-608aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EAYKSEIAHR YNDLGEEHFR GLVLVAFSQY LQQCPFEDHV KLAKEVTEFA KACAAEESGA NCDKSLHTLF GDKLCTVASL RDKYGDMADC CEKQEPDRNE CFLAHKDDNP GFPPLVAPEP DALCAAFQDN EQLFLGKYLY EIARRHPYFY APELLYYAQQ YKGVFAECCQ AADKAACLGP KIEALREKVL LSSAKERFKC ASLQKFGDRA FKAWSVARLS QRFPKADFAE ISKVVTDLTK VHKECCHGDL LECADDRADL AKYMCENQDS ISTKLKECCD KPVLEKSQCL AEVERDELPG DLPSLAADFV EDKEVCKNYQ EAKDVFLGTF LYEYARRHPE YSVSLLLRLA KEYEATLEKC CATDDPPTCY AKVLDEFKPL VDEPQNLVKT NCELFEKLGE YGFQNALLVR YTKKAPQVST PTLVEVSRKL GKVGTKCCKK PESERMSCAE DFLSVVLNRL CVLHEKTPVS ERVTKCCSES LVNRRPCFSG LEVDETYVPK EFNAETFTFH ADLCTLPEAE KQVKKQTALV ELLKHKPKAT DEQLKTVMGD FGAFVEKCCA AENKEGCFSE EGPKLVAAAQ AALV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Reference: Molecular characterization of dog albumin as a cross-reactive allergen.Spitzauer S., Schweiger C., Sperr W.R., Pandjaitan B., Valent P., Muehl S., Ebner C., Scheiner O., Kraft D., Rumpold H.J. Allergy Clin. Immunol. 93:614-627(1994). Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.