Serpin B10 (SERPINB10), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 1-397aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-sumostar-tagged. Target Protein Sequence: MDSLATSINQ FALELSKKLA ESAQGKNIFF SSWSISTSLT IVYLGAKGTT AAQMAQVLQF NRDQGVKCDP ESEKKRKMEF NLSNSEEIHS DFQTLISEIL KPNDDYLLKT ANAIYGEKTY AFHNKYLEDM KTYFGAEPQP VNFVEASDQI RKDINSWVER QTEGKIQNLL PDDSVDSTTR MILVNALYFK GIWEHQFLVQ NTTEKPFRIN ETTSKPVQMM FMKKKLHIFH IEKPKAVGLQ LYYKSRDLSL LILLPEDING LEQLEKAITY EKLNEWTSAD MMELYEVQLH LPKFKLEDSY DLKSTLSSMG MSDAFSQSKA DFSGMSSARN LFLSNVFHKA FVEINEQGTE AAAGSGSEID IRIRVPSIEF NANHPFLFFI RHNKTNTILF YGRLCSP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Protease inhibitor that may play a role in the regulation of protease activities during hematopoiesis and apoptosis induced by TNF. May regulate protease activities in the cytoplasm and in the nucleus. Reference: "Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane: correlation with transcriptome profiling of neutrophil precursors." Rorvig S., Ostergaard O., Heegaard N.H., Borregaard N. J Leukoc Biol 94:711-721(2013). Function: nan