Serotransferrin (Tf), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 20-698aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-KSI-tagged. Target Protein Sequence: VPDKTVKWCA VSEHENTKCI SFRDHMKTVL PADGPRLACV KKTSYQDCIK AISGGEADAI TLDGGWVYDA GLTPNNLKPV AAEFYGSLEH PQTHYLAVAV VKKGTDFQLN QLQGKKSCHT GLGRSAGWII PIGLLFCNLP EPRKPLEKAV ASFFSGSCVP CADPVAFPQL CQLCPGCGCS PTQPFFGYVG AFKCLRDGGG DVAFVKHTTI FEVLPQKADR DQYELLCLDN TRKPVDQYED CYLARIPSHA VVARNGDGKE DLIWEILKVA QEHFGKGKSK DFQLFGSPLG KDLLFKDSAF GLLRVPPRMD YRLYLGHSYV TAIRNQREGV CPEGSIDSAP VKWCALSHQE RAKCDEWSVS SNGQIECESA ESTEDCIDKI VNGEADAMSL DGGHAYIAGQ CGLVPVMAEN YDISSCTNPQ SDVFPKGYYA VAVVKASDSS INWNNLKGKK SCHTGVDRTA GWNIPMGLLF SRINHCKFDE FFSQGCAPGY KKNSTLCDLC IGPAKCAPNN REGYNGYTGA FQCLVEKGDV AFVKHQTVLE NTNGKNTAAW AKDLKQEDFQ LLCPDGTKKP VTEFATCHLA QAPNHVVVSR KEKAARVSTV LTAQKDLFWK GDKDCTGNFC LFRSSTKDLL FRDDTKCLTK LPEGTTYEEY LGAEYLQAVG NIRKCSTSRL LEACTFHKS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Reference: "Role of the basic helix-loop-helix transcription factor, scleraxis, in the regulation of Sertoli cell function and differentiation." Muir T., Sadler-Riggleman I., Skinner M.K. Mol. Endocrinol. 19:2164-2174(2005). Function: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.