Serine/threonine-protein kinase RIO3 (RIOK3), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-519aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE KKFNGDSKVS ISFENYRKVH PYEDSDSSED EVDWQDTRDD PYRPAKPVPT PKKGFIGKGK DITTKHDEVV CGRKNTARME NFAPEFQVGD GIGMDLKLSN HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL MYKMVNSGML ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV LLKKHILVMS FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC TLVHADLSEY NMLWHAGKVW LIDVSQSVEP THPHGLEFLF RDCRNVSQFF QKGGVKEALS ERELFNAVSG LNITADNEAD FLAEIEALEK MNEDHVQKNG RKAASFLKDD GDPPLLYDE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway. May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA. Reference: "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Function: nan